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| 马铃薯Shaker基因家族全基因组鉴定和分析 |
| Genome-wide analysis of the Shaker-type potassium channel gene family in Potato (Solanum tuberosum L.) |
| 投稿时间：2019-03-06 修订日期：2019-03-06 |
| DOI： |
| 中文关键词: Shaker基因家族，马铃薯，钾离子吸收，表达模式，系统发育 |
| 英文关键词: Shaker-type potassium channels, potato, potassium uptake, expression pattern, phylogenetic |
| 基金项目:国家自然科学基金青年科学基金 雷帕霉素靶蛋白调控马铃薯钾素吸收的分子机制研究；马铃薯 MYB 转录因子在植物抗旱中的分子机制研究 |
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| 中文摘要: |
| 钾是植物中含量最丰富的阳离子，对植物的生长发育至关重要。有研究表明，Shaker家族基因在植物钾离子吸收和转运中起着至关重要的作用。然而，目前对于马铃薯中的Shaker基因知之甚少。研究中对马铃薯Shaker基因家族进行了全基因组分析，共鉴定出9个StShaker基因，并根据它们在染色体上的位置将其命名为StShaker1到StShaker9。系统发育分析表明，StShaker基因能够分为5组。蛋白质多序列比对和基序组成分析出了四个保守的结构域，所有的StShaker基因在结构域I都有一个核心的GYGD/E基序。RT-PCR结果显示，低钾胁迫对大多数StShaker基因的在转录水平上没有显著影响。 |
| 英文摘要: |
| Potassium, the most abundant cation in plants, is essential for plant growth and development. It has been suggested that Shaker-type genes play a crucial role in absorbing and transporting potassium. However, little is known about Shaker-type genes in potato. We conducted a comprehensive analysis on the Shaker gene family in potato. A total of 9 putative StShaker genes were identified and designated as StShaker1 to StShaker9 based on their location on chromosomes. Phylogenetic analysis suggested StShaker genes were clustered into five groups. Multiple sequence alignment and motif composition showed the distribution of four conserved domains and all StShaker genes had a core GYGD/E motif in domain I. qRT-PCR results showed, transcript levels of most StShaker genes were not significantly affected by low potassium stress. |
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